Sysmex Journal International
Haemopoietic Progenitor Cell ( HPC ) Counts on the Sysmex XE-2100: A New Tool for Peripheral Blood Stem Cell ( PBSC ) Harvest Monitoring
Florence CYMBALISTA and Remi LETESTU
Hôpital Necker-Enfants Malades, Hôpital Hotel-Dieu
A crucial point in optimising peripheral blood stem cell ( PBSC ) collection is determining precisely when apheresis has to begin. The decision to harvest PBSC is frequently based on the enumeration of CD34+ cells by flow cytometry. The method is expensive, complex, time consuming and creates organizational difficulties as regards bed occupancy and staff availability. Awaiting the result of the CD34+ assay frequently delays the harvesting procedure.
The aim of this study was to evaluate an alternative method for circulating haemopoietic stem cell quantification, namely by means of the Haemopoietic Progenitor Cell ( HPC ) parameter generated by the Sysmex XE-2100 automated haematology analyser. The objective was to determine how HPC could be introduced into clinical practice to minimise the number of CD34 determinations routinely performed, determine its cut-off value for predicting a good yield and evaluate its overall impact and cost.
Haemopoietic Progenitor Cell, HPC, Automated Haematology Analyzer, XE-2100, Peripheral Blood Stem Cell, PBSC
This article is re-printed from the "Proceedings of the Sysmex European Symposium 2003" pp.131-139.