Increased Accuracy of HPC Quantification using the XE-HPC Master Technology

• Increased Accuracy of HPC Quantification using the XE-HPC Master Technology

AUTHOR(S)

F. STÖLZEL^＊１, U. OELSCHLÄGEL^＊１, K. HÖLIG^＊２, G. EHNINGER^＊１, M. BORNHÄUSER^＊１, and C. THIEDE^＊１,３.

*1 Medizinische Klinik und Poliklinik I, Universitätsklinikum Carl Gustav Carus an der     Technischen Universität
*2 Abt. f. Transfusionsmedizin, Universitätsklinikum Carl Gustav Carus an der Technischen     Universitat
*3 Medizinische Klinik und Poliklinik I, Universitätsklinikum Carl Gustav Carus an der     Technischen Universität

SUMMARY

We have evaluated the performance of the XE-HPC Master technology, an improved procedure for the quantification of haematopoietic progenitor cells ( HPC ) on the Sysmex XE-2100 automated haematology analyser.HPC values were determined in twenty healthy donors undergoing apheresis for peripheral blood stem cell collection using the standard HPC method ( HPC res ) and the improved method ( XE-HPC Master ) in parallel on two independent XE-2100 haematology analysers.The results indicate a substantially improved coefficient of variation using the XE-HPC Master ( median 11%, range 5.1 - 30.2% ) compared to the standard research HPC measurements ( median 19.5%, range 4.2 - 35.7% ) ( p < .0001 ).Comparison with CD34 cell counts showed similar correlation coefficients for HPC values determined by both methods; however, the sample size is definitely too small to draw any conclusions.HPC values were found to decrease significantly over time when the samples were kept at room temperature.Dilution experiments did not indicate any effect of high WBC counts on the HPC determination.In conclusion, the XE-HPC Master technology offers significantly improved accuracy of HPC determination.

KEY WORDS

HPC, CD34, Stem Cell Mobilization