Sysmex Journal International

2019Vol.29 No.1


Efficacy of Antimicrobial Agent to Treat Acute Uncomplicated Cystitis through Estimation of Causative Bacterial Group from Bacterial Scattergrams by Using Fully Automated Urine Particle Analyzer UF-1000i


Hideo OZAWA *1,4, Yoshiyuki MIYAJI *2, Naoko YAJIMA *3, Hiroshi YAKUSHIJI *1 and Atsushi NAGAI *2

*1 Department of Urology, Kawasaki Medical School General Medical Center
*2 Department of Urology, Kawasaki Medical School Hospital
*3 Clinical laboratory, Kawasaki Medical School General Medical Center
*4 Department of Urology, Mizushima Central Hospital


Objectives: Currently, physicians can identify bacterial group causing infection, only when the results of urine culture become available, a few days after initial consultation. In the present study, patients with acute uncomplicated cystitis were prescribed antimicrobial agents according to a developed bacteria morphology information (BACT- Info.) program by analyzing the bacterial scattergram on the UF-1000i (UF-1000i; Sysmex Corporation, Kobe, Japan.) This parameter is research use only and cannot be used for diagnosis.

Methods: Urine samples from a total of 40 females with cystitis were examined separately by BACT-Info., bacterial culture and Gram staining. When BACT-Info. flagged the sample with the IP Message “Rods?”, the patients were treated with cefdinir. For the patients whose sample flagged with the IP Message “Cocci/mix?,” levofloxacin was administered.

Results: The sensitivity of BACT-Info. for “Rods?” was high, as 95.8% cases of bacillus were identified. When BACT-Info. demonstrated “Rods?”, bacilli were suspected as causative bacteria, and the patients were treated by cefdinir with 91.7% efficacy. On the other hand, when BACT-Info. reported “Cocci/mix?”, cocci and/or mixed bacterial infection was suspected, and levofloxacin with 57.1% efficacy (less effective than “Rods?” [p = 0.008]) was administered. The BACT-Info. results indicate that this Scattergram Software may be easier/faster in obtaining data compared to urine bacterial culture, as urine culture may grow different types of contaminated bacteria in some cases.

Conclusions: When the BACT-Info. IP Message displayed “Rods?”, cystitis with bacillus was suspected and the patient could be fully treated by short term oral administration of antimicrobial agents. When the program demonstrated “Cocci/mix?”, some patients could not be fully treated with ordinary antimicrobial agents as there are resistant bacteria in Cocci/mix. The Sysmex BACT-Info IP Messaging Software can be used as a guide to select appropriate antimicrobial agents for treating patients on the day of first visit.


Antimicrobial Agent, Bacterial Class Estimation, Bacterial Culture, Urinary Tract Infection, Urine Particle Analyzer


This article is based on the rules of author's facility in Japan. (as of Oct. 2018)
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