Innovative Technologies in the Evaluation of the Neutrophil Functional Activity in Sepsis

• Innovative Technologies in the Evaluation of the Neutrophil Functional Activity in Sepsis

AUTHOR(S)

M. USTYANTSEVA, O. I. KHOKHLOVA and V. V. AGADZHANYAN

Regional Clinical Center of Miners’ Health Protection

SUMMARY

The study purpose was to evaluate the possibility of using indicators of the functional activity of neutrophils in automated hematological analysis in critically ill patients for the diagnosis of septic complications.

Methods: Twenty patients were examined (14/6 men/women, 56 ± 3.8 years). Patients with documented sepsis were enrolled in the main group (n = 10). The comparison group consisted of patients without sepsis (n = 10). As control values, the results of the examination as part of medical checkup of 20 conditionally healthy people aged 45 to 60 years were used.
Samples of peripheral venous blood were examined on a hematological analyser Sysmex XN-1000 (Sysmex Co., Japan). The main parameters including leukocyte count, absolute and relative number of neutrophils, immature granulocytes (IG), and also advanced parameters of inflammation (NEUT-GI - neutrophil granularity intensity, NEUT-RI - neutrophil reactivity) were assessed. Concentrations of C-reactive protein (CRP) and procalcitonin (PCT) were determined using the Cobas 6000 (Roche, Switzerland).
Statistical processing of the results was carried out using the IBM SPSS Statistics 20 software.

Results: Analysis of the results of the expanded parameters of inflammation showed that NEUT-RI in the patients of the main group significantly exceeded both the control values and those observed in the comparison group (by 44.2%, p < 0.001 and 31.2%, p < 0.001, respectively). Subsequent correlation analysis revealed a strong direct correlation between the levels of NEUT-RI and generally recognized biomarkers of sepsis including PCT, CRP (Spearman’s rank correlation coefficients were, respectively, p = 0.683, p = 0.001 and p = 0.714, p < 0.001) in patients in critical condition, which indicates the potential significance of NEUT-RI as a marker of sepsis.

Conclusion: Thus, significant differences in neutrophil fluorescence intensity (NEUT-RI) in critically ill patients with sepsis and without sepsis have been established, which allows considering this parameter as a promising diagnostic marker of sepsis. Simplicity, accessibility and speed of obtaining the result in a standard blood test open up opportunities for its widespread clinical use.

KEY WORDS

Sepsis, the Functional Activity of Neutrophils, Neutrophil Granularity Intensity (NEUT-GI), Neutrophil Reactivity (NEUT-RI),C-Reactive Protein, Procalcitonin.

NOTE(S)

This article is based on current regulatory requirements in EMEA Region. (as of Mar. 2019)
The specifications, performances and functions described here may be different depending on the regions or the countries due to the regulatory affairs, legal matters or local guidelines. For more details, please contact your regional affiliates or distributors.
NEUT-GI − neutrophil granularity intensity, NEUT-RI − neutrophil reactivity intensity, these items are available only in European market.