Introduction and Evaluation of Light Transmission — Platelet Aggregation Method on the Sysmex CS-series Automated Coagulation Analyzer

• Introduction and Evaluation of Light Transmission - Platelet Aggregation Method on the Sysmex CS-series Automated Coagulation Analyzer

AUTHOR(S)

Tasuku SAKAYORI^*１, Yuri WATANABE^*１, Kyoko NAKAJIMA^*１, Eri MISAWA^*１,
Katsushi KOBAYASHI^*２, Hiroshi KURONO^*２, Nobuo ARAI^*１, and Hidenari TAKAOKA^*１

*1 Sysmex Corporation R&D group Hemostasis Product Engineering
*2 HYPHEN BioMed SAS

SUMMARY

Light transmission aggregometry (LTA) is known as a gold standard method of platelet aggregation testing. However, it has been mainly measured by dedicated, semi-automated analyzers. The fully automated Sysmex CS-series coagulation analyzers have recently been upgraded with new software to perform platelet aggregation tests, and permits access to more accurate results.

In this report, we have evaluated performance of platelet aggregation tests on the CS-series with new software; aggregation imprecision, on-board stability of the agonists and reference intervals using the following agonists (reagents): Revohem™ ADP (2 μM), Revohem™ Collagen (2 μg/mL), Revohem™ Epinephrine (5 μM), Revohem™ Arachidonic acid (1 mM) and Revohem™ Ristocetin (1.2 mg/mL). Platelet agonists were adjusted according to the recommendations of SSC/ISTH.

Aggregation imprecision for maximal aggregation (%) was CV 5% or less in normal samples and CV 10% or less in abnormal samples. On-board stability of the agonists was until 10 hours on a CS analyzer. The reference intervals for each agonist was 60% or more.

The CS-series with new software is unique in its ability to perform both automated platelet aggregation and coagulation tests. These instruments have already been installed in clinical laboratories in different countries. The ability to perform platelet aggregation tests on this routine coagulation analyzer will allow access for more clinical laboratories to generate highly standardized platelet function test results.

KEY WORDS

Platelet Aggregation, LTA, CS-series, Revohem™

NOTE(S)

This article is translated and republished from the Sysmex Journal Web Vol.17 No.1, 2016. (Japanese)