Sysmex Journal International
2014Vol.24 No.1
RepublishedYoshie ICHIYANAGI
Central Laboratory, Gifu Municipal Hospital
The guideline for hematuria diagnosis was disclosed in March 2006. It has been defined as diagnostic criteria for hematuria that 5 or more red blood cells/HPF( high-power field, × 400 ) in the urinary sediment sample under microscopy and/or 20 or more red blood cells/μL by using flow cytometry technique with non-centrifuged urine sample are detected.
One HPF of microscopy for urinary sediment is theoretically equivalent to 0.45μL of non-centrifuged urine sample. However, in fact, there is discrepancy between theory and practice for some reasons. In this study, we examined the variance using KOVA slide.
We counted the urinary formed elements of primitive urine and urinary sediment. The results showed the tendency for the small elements to have greater discrepancy than large elements between theory and practice. It might well suggest that the small elements are easy to remain in the supernatant and to be adsorbed to the tube wall through a centrifugal process.
In our results, one HPF of microscopy for urinary sediment was equivalent to 0.31μL for squamous epithelial cells, 0.20μL for white blood cells, and 0.20μL for red blood cells.
This result meets the proportion of 20RBC/μL of primitive urine to 5RBC/HPF under microscopy in the diagnostic criteria for hematuria.
Non-Centrifugal Urine, Urine Sediment, Field Volume, KOVA Slide, SEKISUI Plate
This article is translated and republished from the Sysmex Journal Web Vol. 8 No. 2, 2007. (Japanese)