Sysmex Journal International
The Possibility of the Bacterial Class Estimate Using Urine from Patients with the Urinary Tract Infection by the Fully Automated Urine Particle Analyzer UF-1000i
Tetsuro MURATANI*１,２, Tomoko KOBAYASHI*１,２, Yuki MINAMOTO*１,２, Yoko IKUNO*１,２ and Shinobu MIGITA*２
*1 Kyurin Corporation
*2 Hibiki Research Group for Clinical Microbiology
We investigated bacterial counts and bacterial discrimination in urine samples from patients with urinary tract infection, and compared Sysmex UF-1000i with standard culture method. This study investigated 208 urine samples from different patients. Regarding bacterial counts in urine, these two methods had good correlation, however, the results of the UF-1000i generally showed 10 times higher than culture results method generally. Five of 15 samples that showed result of no growth by culture method and more than 104 cfu/mL by UF-1000i were positive for bacteria by microscopy. This outcome may be attributed to the use of antibiotics or existing bacteria that were unable to grow on blood agar or Drigalski agar.
Scattergram in bacterial channel of UF-1000i was a graph drawn at Y-axis front airlight width, X-axis front airlight strength, and I pulled a straight line centrally and measured the angle from this X-axis by a protractor.
In the study using bacterial suspensions of identified organisms, the angle of the gram-positive cocci and rods were distributed around 40 degrees, and that of the gram-negative cocci and rods were distributed around 20 degrees. Therefore these results suggest the possibility that one can distinguish gram-negative bacteria from gram-positive bacteria by an angle with the X-axis of scattergram of UF-1000i .
In results using clinical specimens, when the angle of the scattergram was less than 30 degrees, the probability that gram-negative bacterium were present was 89.2%. In contrast, when the angle of the scattergram was more than 30 degrees, the probability gram-positive bacterium was 55.8%. Considering that samples with a scattergram angle of more than 30 degrees accounted for 83.3% when isolating only gram-positive bacteria, it is considered that scattergram analysis of UF-1000i might be useful in choosing appropriate antibiotic therapy.
Urinary Tract Infection, Causative Organism, Gram-stain, UF-1000i
This article is translated and republished from Sysmex J. 2010; 33: 87-96.