Comparisons of the Bact Scattergram Pattern by Fully Automated Integrated Urine Analyzer UX-2000 and Microscopic Examination Results Using Gram Stain

• Comparisons of the Bact Scattergram Pattern by Fully Automated Integrated Urine Analyzer UX-2000 and Microscopic Examination Results Using Gram Stain

AUTHOR(S)

Yumi YASUTAKE^*１, Miki HIGUCHI^*１, Shintaro ODA^*１, Yuki TAMURA^*１, Teizou SHIMADU^*１, Hideyuki KOBAYASHI^*２ and Eiji HANAOKA^*１

*1 Saiseikai Yahata General Hospital
*2 Scientific Research Division, Scientific Affairs, Sysmex Corporation

SUMMARY

UX-2000 ( Sysmex ) is fully automated integrated urine analyzer, combining the automated urine cell analyzer and the urine chemistry ( urine test strips ) analyzer. One of the major advantages of this instrument is quantitative bacteria analysis by the specifically optimized detection unit for the bacteria.

Here, we have examined the relation between bacteria scattergram pattern by UX-2000 and microscopic examination results using gram stain then we found that location of dots on the scattergram of UX-2000 tends to be lower part when gram negative bacteria is measured. By analyzing the shape of the cluster of dots on the scattergram ( bact cluster ) with measuring the slope and width, the bact cluster with broad shape was found in the case of the mixture of gram positive and negative bacteria. Accordingly it was suggested to be possible to make an assumption to discriminate between gram positive and negative bacteria by using bact cluster analysis.

KEY WORDS

UX-2000, Bact Scattergram, Gram Stain, Broad Shaped Bact Cluster

NOTE(S)

This article is translated and republished from Sysmex Journal Web Vol.13 Suppl.2 2012.