Comparisons of the Fully Automated Urine Particle Analyzer (UF-1000i) to Quantitative Urine Culture and Nitrite Reaction in Urinary Tract Infection Test

• Comparisons of the Fully Automated Urine Particle Analyzer (UF-1000i) to Quantitative Urine Culture and Nitrite Reaction in Urinary Tract Infection Test

AUTHOR(S)

Sayaka FUJINAMI, Satomi INAMITSU, Chiemi TOKUSHIGE, Hisae YOSHIMURA,
Yuko FUTATA, Hironobu KAWASHIMA and Akira MATSUNAGA

Central Clinical Laboratry, Fukuoka University Hospital

SUMMARY

A flow cytometry-based automated urine analyzer, the UF-1000i  is a device that can measure the concentrations of red blood cells ( RBC ), white blood cells ( WBC ), epithelial cells ( EC ), casts and bacteria in non-centrifuged urine samples. In the present study, the results obtained with the UF-1000i  were compared with those obtained by conventional quantitative urine culture and nitrite reaction in urine. In addition, we investigated whether the scattergram pattern of the UF-1000i  could distinguish between cocci and bacilli.

The results of the UF-1000i  and those of conventional quantitative urine culture correlated well, and sensitivity and specificity for bacteriuria by the UF-1000i  were 96.7% and 68.1%, respectively. The positive rate for nitrite reaction in bacteriuria ( >10⁵/mL )which was measured by the UF-1000i  was 12.7%, and the large part of detected species was Escherichia coli . The concordance rates of the scattergram pattern of the UF-1000i  for bacilli and cocci were 94.7% and 82.7%, respectively. In bacteriuria ( >10⁵/mL ), the scattergram pattern could distinguish between cocci and bacilli.

KEY WORDS

Fully Automatic Urine Analyzer, UF-1000i , Nitrite, Urinary Tract Infection Test

NOTE(S)

This article is translated and republished from Sysmex J. 2011; 34 (1): 48-52.