Sysmex Journal International
2013Vol.23 No.1
RepublishedNobuko KANNO, Makoto KANEKO, Akiko SUZUKI and Yutaka YATOMI
Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo
The fully-automated coagulation analyzer CS-2000i ( hereinafter CS-2000i ; Sysmex Corporation, Kobe, Japan ) is capable of monitoring reactions using four measuring technologies - clotting, chromogenic, immunologic and agglutination methods. The fundamental performance and precision of the Berichrom FXIII chromogenic ammonia release assay on this analyzer is evaluated and reported here. High reproducibility of this assay ( Control Plasma N: mean 83.6%, CV 1.8% ; Control Plasma P: mean 27.3%, CV 4.2% ) and the acceptable linearity studies using pooled plasma ( 14.4-147% ) were obtained. The on-board stability of reagents was demonstrated. Not only the interference material ( Interference Check A Plus ) but also ammonia ( acceptable range of concentration described in the product document ) had no influence on the Berichrom FXIII assay. With this reagent, there seems to be no difference between analyzers ( CS-2000i and Hitachi H-7180 ) and there was good correlation with the FXIII-A subunit antigen. This study suggests that the basic performance of the FXIII activity assay on CS-2000i is favorable and useful as a diagnostic test.
Fully-Automated Coagulation Analyzer, CS-2000i , Berichrom FXIII, Factor XIII Activity
This article is translated and republished from Sysmex Journal Web 12(1), 2011.