Nagisa NAKAZAWA＊１, Noriko WADA＊１, Yuzo TANAKA＊１, Kazumi GONDO＊１,
Yumiko TANAKA＊１, Takayuki SETO＊１, Yoshiko MARUKI＊２, Tamiaki KONDO＊２,
Satomi ASAI＊３, Hiromichi MATSUSHITA＊３ and Hayato MIYACHI＊３
*1 Department of Clinical Laboratory, Tokai University hospital
*2 Scientific Affairs, Sysmex Corporation
*3 Department of Laboratory Medicine, Tokai University School of Medicine
We evaluated the basic assay performance of the body fluid ( BF ) mode on the automated hematology analyzer XN-2000 ( Sysmex ), which is used for analysis of body and punctured fluids. We examined the within-run reproducibility study with 5 or 10 continuous measurements. The coefficients of variation ( CV% ) of nucleated cell count were 2.7 – 16.0% for cerebrospinal fluid ( CSF ), 5.6% for pleural fluid, 2.0% for ascites, 3.8% for synovial fluid, 0.8 – 25.1% for continuous ambulatory peritoneal dialysis ( CAPD ) effluent and 5.0% for bronchoalveolar lavage fluid ( BALF ). The within-run reproducibility was comparatively good in the differential cell counts, although the CV% was relatively high in samples with low number of nucleated cells and for cells with a low appearance ratio. Dilution linearity confirmed a wide range of 0.01-100 × 103/µL. The correlation between the manual method and XN-2000 analysis for nucleated, polymorphonuclear and mononuclear cells was also studied using samples of CSF ( 63 samples ), pleural fluid ( 16 ), synovial fluid ( 7 ), and CAPD effluent ( 21 ). The coefficients of correlation ( r ) for CSF with the nucleated cell count ≥ 100/µL were respectively 0.99, 0.87 and 0.86 for nucleated, polymorphonuclear and mononuclear cells, and those for CSF with the nucleated cell count < 100/µL were respectively 0.85, 0.77 and 0.84. These coefficients of correlation were respectively 1.00, 0.94 and 0.95 for pleural fluid, 0.93, 0.86 and 0.82 for synovial fluid, and 0.99, 0.71, and 0.62 for CAPD effluent. The correlation was high in order of nucleated, polymorphonuclear, and mononuclear cells. The WDF scattergrams for differentials of samples containing abnormal cells showed leukemic cells plotted in the mononuclear cell ( MN ) area, and cancer cells of epithelial cell origin appeared in the high fluorescence intensity ( HF-BF ) area. The nucleated cell count and cell classification of body and punctured fluids by the BF mode on XN-2000 were thought to be useful for examination of routine laboratory, as well as for that on day and night duty.
Body Fluids, Punctured Fluids, Automated Hematology Analyzer, XN-2000, Body Fluid Mode