Estimation of the Causative Bacterial Group from Bacterial Scattergrams of the Fully Automated Urine Particle Analyzer UF-1000i

• Estimation of the Causative Bacterial Group from Bacterial Scattergrams of the Fully Automated Urine Particle Analyzer UF-1000i

AUTHOR(S)

Hideo OZAWA^＊１, Naoko YAJIMA^＊２ and Hideyuki KOBAYASHI^＊３

*1 Department of Urology, Kawasaki Hospital, Kawasaki Medical School
*2 Clinical Laboratory, Kawasaki Hospital, Kawasaki Medical School
*3 Scientific Research Division, Scientific Affairs, Sysmex Corporation

SUMMARY

We analyzed bacterial scattergrams generated by a Sysmex UF-1000i  for estimating whether the causative micro-organisms were  bacilli, cocci, or both.

We classified the distribution patterns of the scattergrams of 81 urine samples found positive in bacterial culture, among those submitted for flow cytometric urinalysis to the Kawasaki Hospital clinical laboratory. The separation line between " High Angle pattern " and " Low Angle pattern " had an angle of 30° from x-axis. Some cases had dots distributed widely with no relationship with the 30° line. This was defined as "Wide pattern". The incidence of the Low Angle pattern, High Angle pattern and Wide pattern was 57%, 20% and 23%, respectively. Most samples having the Low Angle pattern showed bacilli in culture ( 89% ), and those with the High Angle pattern generally showed growth of cocci ( 69% ). Multi-drug resistant bacteria ( e.g., methicillin resistant staphylococcus and extended spectrum b-lactamase producing bacteria ) were found at high incidence ( 32% ) in the Wide pattern samples. Diagnosis of urinary tract infection ( UTI ) is primarily done by microbial culture, which takes a few days to give results. The possibility of estimating the group of the causative bacteria from the distribution angle of the bacterial scattergram could enable prompt diagnosis of complicated UTI and early discontinuation of antibiotic agents in patients with uncomplicated UTI.

KEY WORDS

Urinary Tract Infection, Causative Organisms, UF-1000i , Antibiotic Agents

NOTE(S)

This article is translated and republished from Sysmex J.34 (Suppl.1): 19-26, 2011.