Preliminary Evaluation of Rat Bone Marrow Samples Using the Sysmex XT-2000iV

• Preliminary Evaluation of Rat Bone Marrow Samples Using the Sysmex XT-2000iV

AUTHOR(S)

Mathers RA^＊１, Evans GO^＊１ and Bleby J^＊２

*1 AstraZeneca R+D Charnwood
*2 Sysmex UK, Ltd.

SUMMARY

Accurate and precise examination and classification of animal bone marrow is frequently required for novel preclinical drug evaluation. At present, the techniques employed are often imprecise and labour intensive, requiring highly skilled staff.

We previously evaluated the Automated Hematology Analyzer XT-2000i V ( Sysmex XT-2000i V ) for peripheral blood haematology parameters in three laboratory species. In this study, we examined the use of this haematology analyser to evaluate rat bone marrow suspensions from rat femurs, with comparison to Cytospin^TM preparations and cell counts obtained using flow cytometry.

The Sysmex XT-2000i V was able to perform a total nucleated cell count, cell differential counts and myeloid:erythroid ratios for rat bone marrow suspensions within 45 secs of aspiration. Total nucleated cell counts using the Sysmex XT-2000i V showed good correlation with counts obtained using the Siemens Advia 120^TM.

KEY WORDS

Bone Marrow, Rat Bone Marrow, Sysmex XT-2000i V.