Determining the Time of Harvesting Peripheral Blood Stem Cells Using the HPC, for Monitoring Hematopoietic Progenitor Cells, of the Automated Hematology Analyzer XE-2100

• Determining the Time of Harvesting Peripheral Blood Stem Cells Using the HPC, for Monitoring Hematopoietic Progenitor Cells, of the Automated Hematology Analyzer XE-2100

AUTHOR(S)

K. KAWAKAMI^＊１, Y. ABE^＊１, O. IMATAKI^＊１, Y. TAMAI^＊１, S. HAGIWARA^＊１, T. TOGANO^＊１,
Y. ISHIDA^＊２, K. AMANO^＊２, K. KUSAMA^＊３, E. SHINMORI^＊３, H. YANASE^＊３, N. IKEUCHI^＊４,
T. TODA^＊４, S. KUWABARA^＊４ and Y. SUZUKI^＊４

*1 Stem Cell Transplantation and Hematology Division, Shizuoka Cancer Center Hospital
*2 Department of Pediatrics, Shizuoka Cancer Center Hospital
*3 Department of Infectious Diseases, Shizuoka Cancer Center Hospital
*4 Department of SRL Testing, Shizuoka Cancer Center Hospital

SUMMARY

We have been using the CD34⁺ cell count or the Hematopoietic progenitor cell ( HPC ) count to determine the adequate time to harvest peripheral blood stem cells. HPC counting is convenient way, using the IMI channel of the Automated Hematology Analyzer XE-2100 ( Sysmex, Japan ), with samples taken for CBC. In this study, we analyzed 73 patients of autologous stem cell transplantations or allogenic peripheral blood stem cell donors. Stem cells were harvested from 53 subjects, using a combination of chemotherapy and G-CSF, at a time when the HPC count was 20/μL or more. A total of 77 harvests were performed, sufficient number of CD34⁺ stem cells could be obtained in 69 ( 89.6% ) of these harvests. On an average, 6.04×10⁶/kg CD34⁺ could be obtained per stem cell harvest, which was sufficient for tandem transplantation in myeloma cases. There was positive correlation between the number of CD34⁺ cells harvested and the peripheral blood HPC count. Mobilization was done through G-CSF administration alone in the case of donors. Harvesting was done when the peripheral blood CD34⁺ cell count was 10/μL or more, and the HPC count was also taken simultaneously. Twenty six harvests were done from 16 donors, 1 million or more CD34⁺ cells per kg body weight could be harvested in 23 ( 88.5% ) harvests. There was no significant correlation between the number of harvested CD34⁺ cells and the peripheral blood HPC count ( r=0.346, P=0.0836 ). The peripheral blood CD34⁺ cell count became 10/μL or more in 4 donors on the 4th day of stem cell mobilization, but HPC remained at less than 10/μL, and although their HPC count exceeded 20/μL on the 5th day. Although the time of harvesting could be determined in autologous stem cell harvest using a combination of chemotherapy and G-CSF, it could not be decided based on the HPC count alone in case of allogenic donors.

KEY WORDS

Automated Hematology Analyzer, XE-2100, Stem Cell Harvest, Transplantation, Donor, HPC