Reagent Characteristics in the XE-2100 NRBC Channel

• Reagent Characteristics in the XE-2100 NRBC Channel

AUTHOR(S)

Takashi SAKATA

R&D, Sysmex Corporation

SUMMARY

The measurement of nucleated red blood cell ( NRBCs ) not only provides important information for determining treatment in cases such as thalassemia, etc. but can also give useful information in diagnosing chronic myeloproliferative disorders such as myelofibrosis and chronic myeloid leukemia. However, in the conventional ( manual microscopic ) method, the number of cells counted is usually as few as 100 cells, causing problems in accuracy and precision. In addition, with a typical routine automated hematology analyzer, it is difficult to completely distinguish between NRBC and WBC, and NRBC tends to interfere in the WBC count. To resolve these problems, NRBC counting methods using various FCM have been developed, but few satisfactory results have been obtained.

The XE-2100 has made it possible to accurately count NRBC by using a newly developed NRBC measurment reagent, “ STROMATOLYSER-NR ” and the FCM detector using a red semiconductor laser.

In this report, the NRBC measurement reagent “ STROMATOLYSER-NR ” and the measurement principle of the XE-2100’s NRBC channel will be described. In addition, a new NRBC reference method using FCM will be introduced.

KEY WORDS

Flow Cytometry ( FCM ), Semiconductor Laser, NRBC, Polymethine Dye