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| The RF/DC Impedance Method detects change in impedance of DC (direct current) and RF (radio-frequency current) when a blood cell, in a diluted sample, passes through a small hole (aperture) of the detector. The DC impedance varies with the size (volume) of the blood cell passing through the aperture because the cell functions as an insulator. The RF impedance, on the other hand, changes with the cell's internal characteristics, such as the nucleus size and the density of the cytoplasm. The system uses this information to create a scattergram of the blood cells and to classify each blood cell by its size and internal characteristics. This measurement principle is used in the SE-9000, Fully Automated Hematology Analyzer to differentiate leukocytes into lymphocytes, monocytes and granulocytes and to calculate their numbers and percentages. |
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